|ZFIN ID: ZDB-PUB-140828-7|
Blood Flow and Bmp Signaling Control Endocardial Chamber Morphogenesis
Dietrich, A.C., Lombardo, V.A., Abdelilah-Seyfried, S.
|Source:||Developmental Cell 30: 367-377 (Journal)|
|Registered Authors:||Abdelilah-Seyfried, Salim|
|PubMed:||25158852 Full text @ Dev. Cell|
Dietrich, A.C., Lombardo, V.A., Abdelilah-Seyfried, S. (2014) Blood Flow and Bmp Signaling Control Endocardial Chamber Morphogenesis. Developmental Cell. 30:367-377.
ABSTRACTDuring heart development, the onset of heartbeat and blood flow coincides with a ballooning of the cardiac chambers. Here, we have used the zebrafish as a vertebrate model to characterize chamber ballooning morphogenesis of the endocardium, a specialized population of endothelial cells that line the interior of the heart. By combining functional manipulations, fate mapping studies, and high-resolution imaging, we show that endocardial growth occurs without an influx of external cells. Instead, endocardial cell proliferation is regulated, both by blood flow and by Bmp signaling, in a manner independent of vascular endothelial growth factor (VEGF) signaling. Similar to myocardial cells, endocardial cells obtain distinct chamber-specific and inner- versus outer-curvature-specific surface area sizes. We find that the hemodynamic-sensitive transcription factor Klf2a is involved in regulating endocardial cell morphology. These findings establish the endocardium as the flow-sensitive tissue in the heart with a key role in adapting chamber growth in response to the mechanical stimulus of blood flow.