ZFIN ID: ZDB-PUB-140820-3
A novel HoxB cluster protein expressed in the hindbrain and pharyngeal arches
Saxena, A., Bronner, M.E.
Date: 2014
Source: Genesis (New York, N.Y. : 2000)   52(10): 858-63 (Journal)
Registered Authors: Bronner-Fraser, Marianne, Saxena, Ankur
Keywords: HoxB cluster, Hoxb4a, fluorescent fusion protein, zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/embryology
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/metabolism
  • Bacterial Proteins/metabolism
  • Branchial Region/embryology
  • Branchial Region/metabolism*
  • Cell Nucleus/genetics
  • Cell Nucleus/metabolism
  • Cytoplasm/genetics
  • Cytoplasm/metabolism
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
  • Luminescent Proteins/metabolism
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Rhombencephalon/embryology
  • Rhombencephalon/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 25137177 Full text @ Genesis
We describe a novel zebrafish line that fluorescently tags a previously unknown protein, CT74a, allowing us to follow its endogenous expression in real time and at subcellular resolution in live embryos. Our results showed that CT74a-Citrine fusion protein is expressed in the developing pharyngeal arches, hindbrain, and fin buds in a pattern highly reminiscent of transcription factors belonging to anterior Hox gene families, including expression in a subset of neuronal nuclei. Consistent with this, splinkerette-PCR revealed that CT74a-Citrine's genomic integration is within the HoxB region, and 3' RACE demonstrated that its downstream coding sequence has no recognizable homology. Thus, CT74a is a previously unknown protein located within the HoxB cluster adjacent to Hoxb4a and is expressed in a Hoxb4a-like pattern.