|ZFIN ID: ZDB-PUB-140820-3|
A novel HoxB cluster protein expressed in the hindbrain and pharyngeal arches
Saxena, A., Bronner, M.E.
|Source:||Genesis (New York, N.Y. : 2000) 52(10): 858-63 (Journal)|
|Registered Authors:||Bronner-Fraser, Marianne, Saxena, Ankur|
|Keywords:||HoxB cluster, Hoxb4a, fluorescent fusion protein, zebrafish|
|PubMed:||25137177 Full text @ Genesis|
Saxena, A., Bronner, M.E. (2014) A novel HoxB cluster protein expressed in the hindbrain and pharyngeal arches. Genesis (New York, N.Y. : 2000). 52(10):858-63.
ABSTRACTWe describe a novel zebrafish line that fluorescently tags a previously unknown protein, CT74a, allowing us to follow its endogenous expression in real time and at subcellular resolution in live embryos. Our results showed that CT74a-Citrine fusion protein is expressed in the developing pharyngeal arches, hindbrain, and fin buds in a pattern highly reminiscent of transcription factors belonging to anterior Hox gene families, including expression in a subset of neuronal nuclei. Consistent with this, splinkerette-PCR revealed that CT74a-Citrine's genomic integration is within the HoxB region, and 3' RACE demonstrated that its downstream coding sequence has no recognizable homology. Thus, CT74a is a previously unknown protein located within the HoxB cluster adjacent to Hoxb4a and is expressed in a Hoxb4a-like pattern.