PUBLICATION
Profiling of Biomarkers for the Exposure of Polycyclic Aromatic Hydrocarbons: Lamin-A/C Isoform 3, Poly[ADP-ribose] Polymerase 1, and Mitochondria Copy Number Are Identified as Universal Biomarkers
- Authors
- Kim, H.Y., Kim, H.R., Kang, M.G., Trang, N.T., Baek, H.J., Moon, J.D., Shin, J.H., Suh, S.P., Ryang, D.W., Kook, H., Shin, M.G.
- ID
- ZDB-PUB-140813-1
- Date
- 2014
- Source
- BioMed Research International 2014: 605135 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- Biomarkers/analysis*
- Biomarkers/metabolism
- Cell Line
- Cell Shape/drug effects
- Cell Survival/drug effects
- DNA Copy Number Variations/drug effects*
- DNA Damage/drug effects
- DNA, Mitochondrial/drug effects*
- Embryo, Nonmammalian
- Humans
- Lamins/analysis*
- Lamins/metabolism
- Poly(ADP-ribose) Polymerases/analysis*
- Poly(ADP-ribose) Polymerases/metabolism
- Polycyclic Aromatic Hydrocarbons/toxicity*
- Zebrafish
- PubMed
- 25114913 Full text @ Biomed Res. Int.
Citation
Kim, H.Y., Kim, H.R., Kang, M.G., Trang, N.T., Baek, H.J., Moon, J.D., Shin, J.H., Suh, S.P., Ryang, D.W., Kook, H., Shin, M.G. (2014) Profiling of Biomarkers for the Exposure of Polycyclic Aromatic Hydrocarbons: Lamin-A/C Isoform 3, Poly[ADP-ribose] Polymerase 1, and Mitochondria Copy Number Are Identified as Universal Biomarkers. BioMed Research International. 2014:605135.
Abstract
This study investigated the profiling of polycyclic aromatic hydrocarbon- (PAH-) induced genotoxicity in cell lines and zebrafish. Each type of cells displayed different proportionality of apoptosis. Mitochondrial DNA (mtDNA) copy number was dramatically elevated after 5-day treatment of fluoranthene and pyrene. The notable deregulated proteins for PAHs exposure were displayed as follows: lamin-A/C isoform 3 and annexin A1 for benzopyrene; lamin-A/C isoform 3 and DNA topoisomerase 2-alpha for pentacene; poly[ADP-ribose] polymerase 1 (PARP-1) for fluoranthene; and talin-1 and DNA topoisomerase 2-alpha for pyrene. Among them, lamin-A/C isoform 3 and PARP-1 were further confirmed using mRNA and protein expression study. Obvious morphological abnormalities including curved backbone and cardiomegaly in zebrafish were observed in the 54 hpf with more than 400 nM of benzopyrene. In conclusion, the change of mitochondrial genome (increased mtDNA copy number) was closely associated with PAH exposure in cell lines and mesenchymal stem cells. Lamin-A/C isoform 3, talin-1, and annexin A1 were identified as universal biomarkers for PAHs exposure. Zebrafish, specifically at embryo stage, showed suitable in vivo model for monitoring PAHs exposure to hematopoietic tissue and other organs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping