PUBLICATION
Out with the old, in with the new: reassessing morpholino knockdowns in light of genome editing technology
- Authors
- Schulte-Merker, S., Stainier, D.Y.
- ID
- ZDB-PUB-140808-5
- Date
- 2014
- Source
- Development (Cambridge, England) 141: 3103-4 (Other)
- Registered Authors
- Schulte-Merker, Stefan, Stainier, Didier
- Keywords
- none
- MeSH Terms
-
- Genome
- Zebrafish
- Phenotype
- Morpholinos/genetics*
- Genetic Techniques*/trends
- Mutation
- Animals
- PubMed
- 25100652 Full text @ Development
Citation
Schulte-Merker, S., Stainier, D.Y. (2014) Out with the old, in with the new: reassessing morpholino knockdowns in light of genome editing technology. Development (Cambridge, England). 141:3103-4.
Abstract
Morpholino oligomers have been used widely and for many years in the zebrafish community to transiently knock down the function of target genes. It has often been difficult, however, to reliably discriminate between specific and non-specific effects, and thus generally accepted guidelines to control for morpholino side effects do not exist. In light of recent methodologies to generate mutant lines in virtually any zebrafish gene, we discuss these different approaches with a specific focus on how the first description of a loss-of-function phenotype in zebrafish should be accomplished.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping