PUBLICATION
Fingerprinting of Psychoactive Drugs in Zebrafish Anxiety-Like Behaviors
- Authors
- Maximino, C., da Silva, A.W., Araújo, J., Lima, M.G., Miranda, V., Puty, B., Benzecry, R., Picanço-Diniz, D.L., Gouveia, A., Oliveira, K.R., Herculano, A.M.
- ID
- ZDB-PUB-140801-5
- Date
- 2014
- Source
- PLoS One 9: e103943 (Journal)
- Registered Authors
- Maximino, Caio
- Keywords
- none
- MeSH Terms
-
- Animals
- Anti-Anxiety Agents/pharmacology*
- Anxiety/drug therapy*
- Buspirone/pharmacology
- Caffeine/pharmacology
- Diazepam/pharmacology
- Drug Evaluation, Preclinical
- Freezing Reaction, Cataleptic/drug effects
- Serotonin/metabolism
- Swimming
- Zebrafish
- PubMed
- 25079766 Full text @ PLoS One
Citation
Maximino, C., da Silva, A.W., Araújo, J., Lima, M.G., Miranda, V., Puty, B., Benzecry, R., Picanço-Diniz, D.L., Gouveia, A., Oliveira, K.R., Herculano, A.M. (2014) Fingerprinting of Psychoactive Drugs in Zebrafish Anxiety-Like Behaviors. PLoS One. 9:e103943.
Abstract
A major hindrance for the development of psychiatric drugs is the prediction of how treatments can alter complex behaviors in assays which have good throughput and physiological complexity. Here we report the development of a medium-throughput screen for drugs which alter anxiety-like behavior in adult zebrafish. The observed phenotypes were clustered according to shared behavioral effects. This barcoding procedure revealed conserved functions of anxiolytic, anxiogenic and psychomotor stimulating drugs and predicted effects of poorly characterized compounds on anxiety. Moreover, anxiolytic drugs all decreased, while anxiogenic drugs increased, serotonin turnover. These results underscore the power of behavioral profiling in adult zebrafish as an approach which combines throughput and physiological complexity in the pharmacological dissection of complex behaviors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping