ZFIN ID: ZDB-PUB-140730-10
In vivo imaging of hedgehog pathway activation with a nuclear fluorescent reporter
Mich, J.K., Payumo, A.Y., Rack, P.G., Chen, J.K.
Date: 2014
Source: PLoS One   9: e103661 (Journal)
Registered Authors: Chen, James K., Mich, John, Rack, Paul
Keywords: none
MeSH Terms:
  • Animal Fins/embryology
  • Animal Fins/growth & development
  • Animal Fins/metabolism
  • Animals
  • Animals, Genetically Modified
  • Cell Nucleus/metabolism*
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Hedgehog Proteins/genetics
  • Hedgehog Proteins/metabolism*
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism*
  • Microscopy, Fluorescence
  • Mutation
  • Reproducibility of Results
  • Signal Transduction*
  • Somites/embryology
  • Somites/growth & development
  • Somites/metabolism
  • Time-Lapse Imaging/methods
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 25068273 Full text @ PLoS One
FIGURES
ABSTRACT
The Hedgehog (Hh) pathway is essential for embryonic development and tissue regeneration, and its dysregulation can lead to birth defects and tumorigenesis. Understanding how this signaling mechanism contributes to these processes would benefit from an ability to visualize Hedgehog pathway activity in live organisms, in real time, and with single-cell resolution. We report here the generation of transgenic zebrafish lines that express nuclear-localized mCherry fluorescent protein in a Gli transcription factor-dependent manner. As demonstrated by chemical and genetic perturbations, these lines faithfully report Hedgehog pathway state in individual cells and with high detection sensitivity. They will be valuable tools for studying dynamic Gli-dependent processes in vertebrates and for identifying new chemical and genetic regulators of the Hh pathway.
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