Partial cloning, tissue distribution and effects of epigallocatechin gallate on hepatic 3-hydroxy-3-methylglutaryl-CoA reductase mRNA transcripts in goldfish (Carassius auratus)
- Authors
- Cocci, P., Mosconi, G., and Palermo, F.A.
- ID
- ZDB-PUB-140728-45
- Date
- 2014
- Source
- Gene 545(2): 220-225 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Catechin/analogs & derivatives*
- Catechin/pharmacology
- Cloning, Molecular
- Gene Expression Regulation/drug effects*
- Goldfish/classification
- Goldfish/genetics*
- Goldfish/metabolism*
- Hydroxymethylglutaryl CoA Reductases/chemistry
- Hydroxymethylglutaryl CoA Reductases/genetics*
- Hydroxymethylglutaryl CoA Reductases/metabolism*
- Liver/drug effects*
- Liver/enzymology*
- Molecular Sequence Data
- Organ Specificity
- Phylogeny
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Sequence Alignment
- PubMed
- 24835314 Full text @ Gene
Epigallocatechin gallate (EGCG), the major active component of the green tea, has recently been found to inhibit 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCoAR) activity in vitro and to modulate lipogenesis in vivo. In this study we have evaluated the effects of short-term in vivo exposure to EGCG (6 μg g 1 BW or 9 μg g 1 BW) on hepatic HMGCoAR gene expression of goldfish (Carassius auratus). We initially characterized a partial sequence of goldfish HMGCoAR suggesting that the obtained fragment shares high similarity (> 92%) with other fish HMGCoAR sequences. Further, the HMGCoAR transcript was detected in all goldfish tissues (except muscle) but primarily in liver, brain and gonads; on the contrary, low expression levels were found in intestine, heart, gill, and kidney. Both EGCG doses significantly decreased hepatic HMGCoAR mRNA levels 180 min post-injection. HMGCoAR was also significantly down-regulated at 90 min after injection in fish treated with the highest dose of EGCG. Our results demonstrate that hepatic HMGCoAR gene expression is acutely responsive to short-term EGCG exposure in goldfish. This finding suggests a potential role of EGCG in transcriptional regulation of the rate-limiting enzyme in cholesterol synthesis.