ZFIN ID: ZDB-PUB-140712-16
Calsyntenin-1 Regulates Axon Branching and Endosomal Trafficking during Sensory Neuron Development In Vivo
Ponomareva, O.Y., Holmen, I.C., Sperry, A.J., Eliceiri, K.W., Halloran, M.C.
Date: 2014
Source: The Journal of neuroscience : the official journal of the Society for Neuroscience 34: 9235-48 (Journal)
Registered Authors: Halloran, Mary
Keywords: axon branching, calsyntenin, endosome, polarity, trafficking, zebrafish
MeSH Terms:
  • Aging/pathology
  • Aging/physiology
  • Animals
  • Animals, Genetically Modified
  • Axons/physiology*
  • Axons/ultrastructure*
  • Calcium-Binding Proteins/genetics
  • Calcium-Binding Proteins/metabolism*
  • Cells, Cultured
  • Endosomes/physiology*
  • Endosomes/ultrastructure
  • Models, Animal
  • Neurogenesis/physiology
  • Neuronal Plasticity/physiology*
  • Protein Transport/physiology
  • Sensory Receptor Cells/physiology*
  • Sensory Receptor Cells/ultrastructure*
  • Zebrafish
PubMed: 25009257 Full text @ J. Neurosci.
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ABSTRACT
Precise regulation of axon branching is crucial for neuronal circuit formation, yet the mechanisms that control branch formation are not well understood. Moreover, the highly complex morphology of neurons makes them critically dependent on protein/membrane trafficking and transport systems, although the functions for membrane trafficking in neuronal morphogenesis are largely undefined. Here we identify a kinesin adaptor, Calsyntenin-1 (Clstn-1), as an essential regulator of axon branching and neuronal compartmentalization in vivo. We use morpholino knockdown and a Clstn-1 mutant to show that Clstn-1 is required for formation of peripheral but not central sensory axons, and for peripheral axon branching in zebrafish. We used live imaging of endosomal trafficking in vivo to show that Clstn-1 regulates transport of Rab5-containing endosomes from the cell body to specific locations of developing axons. Our results suggest a model in which Clstn-1 patterns separate axonal compartments and define their ability to branch by directing trafficking of specific endosomes.
ADDITIONAL INFORMATION