PUBLICATION
Involvement of Upregulated SYF2 in Schwann Cell Differentiation and Migration After Sciatic Nerve Crush
- Authors
- Zhou, Z., Liu, Y., Nie, X., Cao, J., Zhu, X., Yao, L., Zhang, W., Yu, J., Wu, G., Liu, Y., Yang, H.
- ID
- ZDB-PUB-140626-5
- Date
- 2014
- Source
- Cellular and molecular neurobiology 34(7): 1023-36 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Biomarkers/metabolism
- Blotting, Western
- CD11b Antigen/metabolism
- Cell Differentiation*/drug effects
- Cell Movement*/drug effects
- Cyclic AMP/pharmacology
- Immunohistochemistry
- Male
- Models, Biological
- Nerve Crush*
- Nuclear Proteins/metabolism*
- Octamer Transcription Factor-6/metabolism
- Phenotype
- RNA, Small Interfering/metabolism
- Rats, Sprague-Dawley
- Schwann Cells/drug effects
- Schwann Cells/metabolism
- Schwann Cells/pathology*
- Sciatic Nerve/pathology*
- Tubulin/metabolism
- Up-Regulation*/drug effects
- PubMed
- 24962097 Full text @ Cell. Mol. Neurobiol.
Citation
Zhou, Z., Liu, Y., Nie, X., Cao, J., Zhu, X., Yao, L., Zhang, W., Yu, J., Wu, G., Liu, Y., Yang, H. (2014) Involvement of Upregulated SYF2 in Schwann Cell Differentiation and Migration After Sciatic Nerve Crush. Cellular and molecular neurobiology. 34(7):1023-36.
Abstract
SYF2 is a putative homolog of human p29 in Saccharomyces cerevisiae. It seems to be involved in pre-mRNA splicing and cell cycle progression. Disruption of SYF2 leads to reduced α-tubulin expression and delayed nerve system development in zebrafish. Due to the potential of SYF2 in modulating microtubule dynamics in nervous system, we investigated the spatiotemporal expression of SYF2 in a rat sciatic nerve crush (SNC) model. We found that SNC resulted in a significant upregulation of SYF2 from 3 days to 1 week and subsequently returned to the normal level at 4 weeks. At its peak expression, SYF2 distributed predominantly in Schwann cells. In addition, upregulation of SYF2 was approximately in parallel with Oct-6, and numerous Schwann cells expressing SYF2 were Oct-6 positive. In vitro, we observed enhanced expression of SYF2 during the process of cyclic adenosine monophosphate (cAMP)-induced Schwann cell differentiation. SYF2-specific siRNA-transfected Schwann cells did not show significant morphological change in the process of Schwann cell differentiation. Also, we found shorter and disorganized microtubule structure and a decreased migration in SYF2-specific siRNA-transfected Schwann cells. Together, these findings indicated that the upregulation of SYF2 was associated with Schwann cell differentiation and migration following sciatic nerve crush.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping