PUBLICATION

Mass Spectrometry for Proteomics-Based Investigation Using the Zebrafish Vertebrate Model System

Authors
Baral, R., Wetie, A.G., Darie, C.C., Wallace, K.N.
ID
ZDB-PUB-140622-3
Date
2014
Source
Advances in experimental medicine and biology   806: 331-340 (Chapter)
Registered Authors
Wallace, Kenneth
Keywords
none
MeSH Terms
  • Animals
  • Genome/physiology*
  • Humans
  • Intestines/metabolism*
  • Mass Spectrometry/methods*
  • Mutation
  • Proteomics/methods*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
24952190 Full text @ Adv. Exp. Med. Biol.
Abstract
The zebrafish (Danio rerio) is frequently being used to investigate the genetics of human diseases as well as resulting pathologies. Ease of both forward and reverse genetic manipulation along with conservation of vertebrate organ systems and disease causing genes has made this system a popular model. Many techniques have been developed to manipulate the genome of zebrafish producing mutants in a vast array of genes. While genetic manipulation of zebrafish has progressed, proteomics have been under-utilized. This review highlights studies that have already been performed using proteomic techniques and as well as our initial proteomic work comparing changes to the proteome between the ascl1a-/- and WT intestine.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping