PUBLICATION
Indole-3-carbinol, a vegetable phytochemical, inhibits adipogenesis by regulating cell cycle and AMPKα signaling
- Authors
- Choi, H.S., Jeon, H.J., Lee, O.H., Lee, B.Y.
- ID
- ZDB-PUB-140622-2
- Date
- 2014
- Source
- Biochimie 104: 127-36 (Journal)
- Registered Authors
- Keywords
- 3T3-L1, AMPKα, Indole-3-carbinol, adipogenesis, zebrafish
- MeSH Terms
-
- 3T3-L1 Cells
- AMP-Activated Protein Kinases/metabolism*
- Adipocytes/cytology
- Adipocytes/drug effects
- Adipocytes/metabolism
- Adipogenesis/drug effects*
- Animals
- Cell Cycle/drug effects*
- Cell Differentiation/drug effects
- Cell Proliferation/drug effects
- Cell Survival/drug effects
- Indoles/pharmacology*
- Mice
- Mitosis/drug effects
- Signal Transduction/drug effects*
- Triglycerides/biosynthesis
- Triglycerides/metabolism
- Vegetables/chemistry*
- Zebrafish
- PubMed
- 24952351 Full text @ Biochimie
Citation
Choi, H.S., Jeon, H.J., Lee, O.H., Lee, B.Y. (2014) Indole-3-carbinol, a vegetable phytochemical, inhibits adipogenesis by regulating cell cycle and AMPKα signaling. Biochimie. 104:127-36.
Abstract
Indole-3-carbinol (I3C) is a phytochemical present mainly in cruciferous vegetables. In this study, we investigated the mechanism by which I3C blocks adipogenesis in 3T3-L1 cells, and evaluated the anti-adipogenic effect of I3C in zebrafish. Our data showed that I3C mainly inhibits early differentiation of adipocyte through cell cycle arrest. Inhibition of early differentiation was reflected by down-regulation of early adipogenic factors such as CCAAT-enhancer binding proteins β and δ (C/EBPβ and C/EBPδ), followed by down-regulation of late adipogenic factors such as peroxisome proliferator-activated receptor γ (PPARγ) and C/EBPα, and regulation of signaling molecules. This result was supported by a reduction in triglyceride (TG) levels and TG synthetic enzymes. I3C activated AMP-activated protein kinase α (AMPKα) to inhibit fatty acid synthesis. In addition, an anti-adipogenic effect of I3C was found in zebrafish study. Our data suggest that vegetables-derived I3C could reduce lipid accumulation via various molecular mechanisms in cell.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping