PUBLICATION
Ror2 Receptor Mediates Wnt11 Signaling and Affects Convergence and Extension Movements in Zebrafish
- Authors
- Bai, Y., Tan, X., Zhang, H., Liu, C., Zhao, B., Li, Y., Lu, L., Liu, Y., Zhou, J.
- ID
- ZDB-PUB-140615-4
- Date
- 2014
- Source
- The Journal of biological chemistry 289(30): 20664-20676 (Journal)
- Registered Authors
- Zhou, Jianfeng
- Keywords
- Wnt signaling, serine/threonine protein kinase, signal transduction, tyrosine-protein kinase (tyrosine kinase), zebrafish
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing/genetics
- Adaptor Proteins, Signal Transducing/metabolism
- Animals
- Cell Movement/physiology*
- Gastrula/cytology
- Gastrula/embryology*
- Gastrulation/physiology*
- Gene Knockdown Techniques
- Mice
- Monomeric GTP-Binding Proteins/genetics
- Monomeric GTP-Binding Proteins/metabolism
- Phosphoproteins/genetics
- Phosphoproteins/metabolism
- Receptor Tyrosine Kinase-like Orphan Receptors/genetics
- Receptor Tyrosine Kinase-like Orphan Receptors/metabolism*
- Wnt Proteins/genetics
- Wnt Proteins/metabolism*
- Wnt Signaling Pathway/physiology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 24928507 Full text @ J. Biol. Chem.
Citation
Bai, Y., Tan, X., Zhang, H., Liu, C., Zhao, B., Li, Y., Lu, L., Liu, Y., Zhou, J. (2014) Ror2 Receptor Mediates Wnt11 Signaling and Affects Convergence and Extension Movements in Zebrafish. The Journal of biological chemistry. 289(30):20664-20676.
Abstract
The receptor tyrosine kinase Ror2 acts as an alternative receptor or coreceptor for Wnt5a and mediates Wnt5a-induced convergent extension movements during embryogenesis in mice and Xenopus, as well as the polarity and migration of several cell types during development. However, little is known about whether Ror2 function is conserved in other vertebrates or is involved in other non-canonical Wnt ligands in vivo. In this study, we demonstrated that overexpression of dominant-negative ror2 (ror2-TM) mRNA in zebrafish embryos resulted in convergence and extension defects and incompletely separated eyes, which is consistent with observations from slb/wnt11 mutants or wnt11 knockdown morphants. Moreover, the co-injection of ror2-TM mRNA and a wnt11 morpholino or the coexpression of ror2 and wnt11 in zebrafish embryos synergetically induced more severe convergence and extension defects. Transplantation studies further demonstrated that the Ror2 receptor responded to the Wnt11 ligand and regulated cell migration and cell morphology during gastrulation. DnRor2 inhibited the action of Wnt11, which was revealed by a decreased percentage of Wnt11-induced convergence and extension defects. Ror2 physically interacts with Wnt11. The intracellular Y647 and S863 sites of Ror2 are essential for mediating the action of Wnt11. Dishevelled and RhoA act downstream of Wnt11-Ror2 to regulate convergence and extension movements. Overall, our data suggest an important role of Ror2 in mediating Wnt11 signaling and in regulating convergence and extension movements in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping