PUBLICATION

R-Spondin 3 Regulates Dorsoventral and Anteroposterior Patterning by Antagonizing Wnt/β-Catenin Signaling in Zebrafish Embryos

Authors
Rong, X., Chen, C., Zhou, P., Zhou, Y., Li, Y., Lu, L., Liu, Y., Zhou, J., Duan, C.
ID
ZDB-PUB-140612-1
Date
2014
Source
PLoS One   9(6): e99514 (Journal)
Registered Authors
Duan, Cunming, Rong, Xiaozhi, Zhou, Jianfeng
Keywords
Embryos, Zebrafish, Wnt signaling cascade, Signal inhibition, Xenopus, Gene expression, Hyperexpression techniques, Elephants
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Body Patterning*
  • Evolution, Molecular
  • Humans
  • Intracellular Signaling Peptides and Proteins/chemistry
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/physiology*
  • Protein Structure, Tertiary
  • Thrombospondins/genetics
  • Thrombospondins/physiology
  • Wnt Signaling Pathway*
  • Zebrafish/embryology
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed
24918770 Full text @ PLoS One
Abstract

The Wnt/β-catenin or canonical Wnt signaling pathway plays fundamental roles in early development and in maintaining adult tissue homeostasis. R-spondin 3 (Rspo3) is a secreted protein that has been implicated in activating the Wnt/β-catenin signaling in amphibians and mammals. Here we report that zebrafish Rspo3 plays a negative role in regulating the zygotic Wnt/β-catenin signaling. Zebrafish Rspo3 has a unique domain structure. It contains a third furin-like (FU3) domain. This FU3 is present in other four ray-finned fish species studied but not in elephant shark. In zebrafish, rspo3 mRNA is maternally deposited and has a ubiquitous expression in early embryonic stages. After 12 hpf, its expression becomes tissue-specific. Forced expression of rspo3 promotes dorsoanterior patterning and increases the expression of dorsal and anterior marker genes. Knockdown of rspo3 increases ventral-posterior development and stimulates ventral and posterior marker genes expression. Forced expression of rspo3 abolishes exogenous Wnt3a action and reduces the endogenous Wnt signaling activity. Knockdown of rspo3 results in increased Wnt/β-catenin signaling activity. Further analyses indicate that Rspo3 does not promote maternal Wnt signaling. Human RSPO3 has similar action when tested in zebrafish embryos. These results suggest that Rspo3 regulates dorsoventral and anteroposterior patterning by negatively regulating the zygotic Wnt/β-catenin signaling in zebrafish embryos.

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Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes