PUBLICATION

Effects of Metronidazole on Proopiomelanocortin a Gene Expression in Zebrafish

Authors
Cheng, X., Chen, X., Li, D., Jin, X., He, J., Yin, Z.
ID
ZDB-PUB-140608-2
Date
2015
Source
General and comparative endocrinology   214: 87-94 (Journal)
Registered Authors
He, Jiangyan, Yin, Zhan
Keywords
Endocrine interference, Metronidazole, Pomca expression, Zebrafish
MeSH Terms
  • Animals
  • Apoptosis/drug effects
  • Blotting, Western
  • Cell Proliferation/drug effects
  • Flow Cytometry
  • Gene Expression Regulation, Developmental/drug effects*
  • Metronidazole/pharmacology*
  • Organ Specificity
  • Pituitary Gland/drug effects*
  • Pituitary Gland/growth & development
  • Pituitary Gland/metabolism
  • Pro-Opiomelanocortin/genetics
  • Pro-Opiomelanocortin/metabolism*
  • RNA, Messenger/genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
24907628 Full text @ Gen. Comp. Endocrinol.
Abstract
The Metronidazole (MTZ), a widely used antibiotic for treating variations of infections, recently is applied in a powerful tool for specifically ablating cells or tissues when combined with E.coli nitroreductase (NTR). Although some undesired biological effects on eukaryote cells have been reported previously, the toxicological mechanism of MTZ has not been uncovered yet. In current study, we found that MTZ can induce proopiomelanocortin a (pomca) expression in zebrafish larvae. The effect of MTZ is in stage-sensitive and dose-dependent manner. A pro-proliferation activity of MTZ on pomca-expressing cells in the pituitary at larval stage was also observed. Furthermore, up-regulated levels of prolactin (prl) and glycoprotein hormone subunit α (gsuα) were also observed after the MTZ treatment. Therefore, utilizing our zebrafish as in vivo model, we concluded that MTZ can interfere the endocrine signals in the pituitary hormone genes expression. Our current results raised the cautions to the intensively application of MTZ in clinical practices and biomedical researches.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes