PUBLICATION
Quantification of larval zebrafish motor function in multiwell plates using open-source MATLAB applications
- Authors
- Zhou, Y., Cattley, R.T., Cario, C.L., Bai, Q., Burton, E.A.
- ID
- ZDB-PUB-140606-2
- Date
- 2014
- Source
- Nature Protocols 9: 1533-1548 (Journal)
- Registered Authors
- Burton, Edward A.
- Keywords
- none
- MeSH Terms
-
- Animals
- Image Processing, Computer-Assisted/instrumentation
- Image Processing, Computer-Assisted/methods*
- Larva
- Motor Activity/physiology*
- Software*
- Video Recording/instrumentation
- Video Recording/methods
- Zebrafish/physiology*
- PubMed
- 24901738 Full text @ Nat. Protoc.
Citation
Zhou, Y., Cattley, R.T., Cario, C.L., Bai, Q., Burton, E.A. (2014) Quantification of larval zebrafish motor function in multiwell plates using open-source MATLAB applications. Nature Protocols. 9:1533-1548.
Abstract
This article describes a method to quantify the movements of larval zebrafish in multiwell plates, using the open-source MATLAB applications LSRtrack and LSRanalyze. The protocol comprises four stages: generation of high-quality, flatly illuminated video recordings with exposure settings that facilitate object recognition; analysis of the resulting recordings using tools provided in LSRtrack to optimize tracking accuracy and motion detection; analysis of tracking data using LSRanalyze or custom MATLAB scripts; and implementation of validation controls. The method is reliable, automated and flexible, requires <1 h of hands-on work for completion once optimized and shows excellent signal:noise characteristics. The resulting data can be analyzed to determine the following: positional preference; displacement, velocity and acceleration; and duration and frequency of movement events and rest periods. This approach is widely applicable to the analysis of spontaneous or stimulus-evoked zebrafish larval neurobehavioral phenotypes resulting from a broad array of genetic and environmental manipulations, in a multiwell plate format suitable for high-throughput applications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping