ZFIN ID: ZDB-PUB-140605-6
A role for transcription factor glial cell missing 2 in Ca(2+) homeostasis in zebrafish, Danio rerio
Kumai, Y., Kwong, R.W., Perry, S.F.
Date: 2015
Source: Pflugers Archiv : European journal of physiology   467(4): 753-65 (Journal)
Registered Authors: Perry, Steve F.
Keywords: none
MeSH Terms:
  • Acclimatization
  • Animals
  • Calcium/metabolism*
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism*
  • Epithelial Cells/metabolism
  • Homeostasis*
  • Hydrogen-Ion Concentration
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • TRPV Cation Channels/genetics
  • TRPV Cation Channels/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 24893788 Full text @ Pflügers Archiv. / Eur. J. Physiol.
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ABSTRACT
The present study investigated the role of the transcription factor, glial cell missing 2 (gcm2), in Ca(2+) regulation in zebrafish larvae. Translational gene knockdown of gcm2 decreased Ca(2+) uptake and the density of ionocytes expressing the epithelial Ca(2+) channel (ecac), and disrupted the overall Ca(2+) balance. Ca(2+) uptake and the expression of gcm2 messenger RNA (mRNA) were significantly elevated in larvae acclimated to low Ca(2+) water (25 μM); the stimulation of Ca(2+) uptake was not observed in fish experiencing gcm2 knockdown. Acclimation to acidic water (pH 4) significantly reduced whole-body Ca(2+) content owing to reduced Ca(2+) uptake and increased Ca(2+) efflux. However, ecac mRNA levels and the density of ecac-expressing ionocytes were increased in fish acclimated to acidic water, and maximal Ca(2+) uptake capacity (J MAX) was significantly increased when measured in control water (pH ~7.4). Acclimation of larvae to acidic water significantly increased gcm2 mRNA expression, and in gcm2 morphants, no such stimulation in Ca(2+) uptake was observed after their return to control water. Overexpression of gcm2 mRNA resulted in a significant increase in the numbers of ecac-expressing ionocytes and Ca(2+) uptake. These observations reveal a critical role for gcm2 in Ca(2+) homeostasis in zebrafish larvae.
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