PUBLICATION
Molecular phylogeny of C1 inhibitor depicts two immunoglobulin-like domains fusion in fishes and ray-finned fishes specific intron insertion after separation from zebrafish
- Authors
- Kumar, A., Bhandari, A., Sarde, S.J., Goswami, C.
- ID
- ZDB-PUB-140601-7
- Date
- 2014
- Source
- Biochemical and Biophysical Research Communications 450(1): 219-26 (Journal)
- Registered Authors
- Keywords
- C1 inhibitor, Intron gain, Phylogenetic analysis, Serpin G1, Synteny, group V4
- MeSH Terms
-
- Animals
- Base Sequence
- Complement C1 Inhibitor Protein/genetics*
- Conserved Sequence
- Evolution, Molecular
- Immunoglobulins/genetics*
- Introns/genetics*
- Molecular Sequence Data
- Mutagenesis, Insertional/genetics*
- Phylogeny
- Protein Structure, Tertiary/genetics
- Skates, Fish/genetics*
- Species Specificity
- Zebrafish/genetics*
- PubMed
- 24878530 Full text @ Biochem. Biophys. Res. Commun.
Citation
Kumar, A., Bhandari, A., Sarde, S.J., Goswami, C. (2014) Molecular phylogeny of C1 inhibitor depicts two immunoglobulin-like domains fusion in fishes and ray-finned fishes specific intron insertion after separation from zebrafish. Biochemical and Biophysical Research Communications. 450(1):219-26.
Abstract
C1IN is the multi-facet serine protease inhibitor in the plasma cascades, inhibiting several proteases, notably, regulates both complement and contact system activation. Despite huge advancements in the understanding of C1 inhibitor based on biochemical properties and its roles in the plasma cascades, phylogenetic history of C1IN remain forgotten. To date, there is no comprehensive study illustrating the phylogenetic history of C1IN. Herein, we explored phylogenetic history of C1IN gene in vertebrates. Fishes have C1IN with two immunoglobulin like domains attached in the N-terminal region. The RCL regions of CIIN from fishes and tetrapod genomes have variations at the positions P2 and P1'. Gene structures of C1IN gene from selected ray-finned fishes varied in the Ig domain region with creation of novel intron splitting exon Im2 into Im2a and Im2b. This intron is limited to ray-finned fishes with genome size reduced below 1 Gb, Hence processes responsible for genome compaction and associated double-strand break repairs are accountable for this intron gain. We found C1IN remain on the same locus for ∼450 MY in 52 vertebrates analysed but it is not found in frogs and lampreys.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping