PUBLICATION
miR-203 regulates progenitor cell proliferation during adult zebrafish retina regeneration
- Authors
- Rajaram, K., Harding, R., Hyde, D.R., Patton, J.G.
- ID
- ZDB-PUB-140527-5
- Date
- 2014
- Source
- Developmental Biology 392(2): 393-403 (Journal)
- Registered Authors
- Harding, Rachel
- Keywords
- Mir-203, Pax6b, Progenitor cell proliferation, Retina regeneration
- MeSH Terms
-
- Animals
- Blotting, Western
- Cell Proliferation*
- Cloning, Molecular
- Electroporation
- Flow Cytometry
- Gene Expression Regulation/genetics*
- Immunohistochemistry
- MicroRNAs/genetics
- MicroRNAs/metabolism*
- Microinjections
- Morpholinos/genetics
- Real-Time Polymerase Chain Reaction
- Regeneration/physiology*
- Retina/physiology*
- Reverse Transcriptase Polymerase Chain Reaction
- Statistics, Nonparametric
- Stem Cells/physiology*
- Zebrafish/genetics
- Zebrafish/physiology*
- PubMed
- 24858486 Full text @ Dev. Biol.
Citation
Rajaram, K., Harding, R., Hyde, D.R., Patton, J.G. (2014) miR-203 regulates progenitor cell proliferation during adult zebrafish retina regeneration. Developmental Biology. 392(2):393-403.
Abstract
Damage of the zebrafish retina triggers a spontaneous regeneration response that is initiated by Müller Glia (MG) dedifferentiation and asymmetric cell division to produce multipotent progenitor cells. Subsequent expansion of the progenitor pool by proliferation is critical for retina regeneration. Pax6b expression in the progenitor cells is necessary for their proliferation, but exact regulation of its expression is unclear. Here, we show that miR-203 is downregulated during regeneration in proliferating progenitor cells. Elevated miR-203 levels inhibit progenitor cell expansion without affecting MG dedifferentiation or progenitor cell generation. Using GFP-reporter assays and gain and loss of function experiments in the retina, we show that miR-203 expression must be suppressed to allow pax6b expression and subsequent progenitor cell proliferation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping