ZFIN ID: ZDB-PUB-140513-82
Fluorescent transgenic zebrafish as a biosensor for growth-related effects of methyl parathion
Almeida, D.V., Vaz, B., Azevedo Figueiredo, M., Junior, A.S., Marins, L.F.
Date: 2014
Source: Aquatic toxicology (Amsterdam, Netherlands)   152C: 147-151 (Journal)
Registered Authors: Almeida, Daniela Volcan, Marins, Luis Fernando
Keywords: Destabilized red fluorescent protein, Pesticide, Transgenesis
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Biomarkers/metabolism*
  • Biosensing Techniques/methods*
  • Environmental Monitoring/methods*
  • Gene Expression Regulation/drug effects
  • Growth and Development/drug effects*
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Methyl Parathion/toxicity*
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish/physiology*
PubMed: 24768855 Full text @ Aquat. Toxicol.
Transgenic fish models are potential alternative subjects in toxicological studies, since they can provide in vivo information on the deleterious effects of different substances. Here, we used a transgenic zebrafish (Danio rerio) lineage, which expresses a destabilized fluorescent protein (DsRED) driven by the myosin light chain promoter (Mylz2), in order to propose a new research tool for environmental biomonitoring. For validating the MYO-RED lineage, we exposed fish to the organophosphorated pesticide methyl parathion (MP). The effect of MP on fish growth was assessed by evaluating weight, length, condition factor and muscle fiber diameter. All factors suffered reduction at both tested concentrations (0.13μM and 13μM of MP). Similarly, fluorescence intensity decreased in a concentration-dependent manner, suggesting muscle protein catabolism. However, DsRED gene expression lowered only at the higher MP concentration. Results indicate that the MYO-RED transgenic zebrafish is an interesting model for detecting the growth-related effects of pollutants. Destabilized proteins such as reporter genes are apparently sensitive biomarkers, since effects were observed even at the lower, environmentally acceptable concentration. Therefore, this transgenic fish is a promising candidate model for sensitive, fast, and easy environmental monitoring.