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ZFIN ID: ZDB-PUB-140513-429
Localization of BDNF expression in the developing brain of zebrafish
De Felice, E., Porreca, I., Alleva, E., De Girolamo, P., Ambrosino, C., Ciriaco, E., Germanà, A., Sordino, P.
Date: 2014
Source: Journal of anatomy   224: 564-74 (Journal)
Registered Authors: Germanà, Antonino, Sordino, Paolo
Keywords: brain-derived neurotrophic factor, central nervous system, development, real-time quantitative polymerase chain reaction, whole-mount in situ hybridization, zebrafish
MeSH Terms:
  • Animals
  • Brain/embryology
  • Brain/growth & development
  • Brain/metabolism*
  • Brain-Derived Neurotrophic Factor/genetics
  • Brain-Derived Neurotrophic Factor/metabolism*
  • Gene Expression Regulation, Developmental
  • In Situ Hybridization
  • Neurons/metabolism
  • RNA, Messenger/genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish
PubMed: 24588510 Full text @ J. Anat.
The brain-derived neurotrophic factor (BDNF) gene is expressed in differentiating and post-mitotic neurons of the zebrafish embryo, where it has been implicated in Huntington's disease. Little is known, however, about the full complement of neuronal cell types that express BDNF in this important vertebrate model. Here, we further explored the transcriptional profiles during the first week of development using real-time quantitative polymerase chain reaction (RT-qPCR) and whole-mount in situ hybridization (WISH). RT-qPCR results revealed a high level of maternal contribution followed by a steady increase of zygotic transcription, consistent with the notion of a prominent role of BDNF in neuronal maturation and maintenance. Based on WISH, we demonstrate for the first time that BDNF expression in the developing brain of zebrafish is structure specific. Anatomical criteria and co-staining with genetic markers (shh, pax2a, emx1, krox20, lhx2b and lhx9) visualized major topological domains of BDNF-positive cells in the pallium, hypothalamus, posterior tuberculum and optic tectum. Moreover, the relative timing of BDNF transcription in the eye and tectum may illustrate a mechanism for coordinated development of the retinotectal system. Taken together, our results are compatible with a local delivery and early role of BDNF in the developing brain of zebrafish, adding basic knowledge to the study of neurotrophin functions in neural development and disease.