ZFIN ID: ZDB-PUB-140513-321
Spatial and temporal control of transgene expression in zebrafish
Akerberg, A.A., Stewart, S., Stankunas, K.
Date: 2014
Source: PLoS One   9: e92217 (Journal)
Registered Authors: Akerberg, Alex, Stankunas, Kryn, Stewart, Scott
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • DNA-Binding Proteins/genetics
  • Green Fluorescent Proteins/biosynthesis
  • Green Fluorescent Proteins/genetics
  • Organ Specificity
  • Peptide Elongation Factor 1/genetics
  • Promoter Regions, Genetic
  • Rabbits
  • Receptor, Notch1/biosynthesis
  • Receptor, Notch1/genetics
  • Receptors, Estrogen/biosynthesis
  • Receptors, Estrogen/genetics
  • Recombinant Fusion Proteins/biosynthesis
  • Recombinant Fusion Proteins/genetics*
  • Tamoxifen/pharmacology
  • Transcription Factors/genetics
  • Transcriptional Activation/drug effects*
  • Transgenes
  • Xenopus Proteins/genetics
  • Xenopus laevis/genetics
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • beta-Globins/genetics
PubMed: 24643048 Full text @ PLoS One
Transgenic zebrafish research has provided valuable insights into gene functions and cell behaviors directing vertebrate development, physiology, and disease models. Most approaches use constitutive transgene expression and therefore do not provide control over the timing or levels of transgene induction. We describe an inducible gene expression system that uses new tissue-specific zebrafish transgenic lines that express the Gal4 transcription factor fused to the estrogen-binding domain of the human estrogen receptor. We show these Gal4-ERT driver lines confer rapid, tissue-specific induction of UAS-controlled transgenes following tamoxifen exposure in both embryos and adult fish. We demonstrate how this technology can be used to define developmental windows of gene function by spatiotemporal-controlled expression of constitutively active Notch1 in embryos. Given the array of existing UAS lines, the modular nature of this system will enable many previously intractable zebrafish experiments.