PUBLICATION
Persistent impaired glucose metabolism in a zebrafish hyperglycemia model
- Authors
- Capiotti, K.M., Antonioli, R., Kist, L.W., Bogo, M.R., Bonan, C.D., Da Silva, R.S.
- ID
- ZDB-PUB-140513-199
- Date
- 2014
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 171C: 58-65 (Journal)
- Registered Authors
- Keywords
- Diabetes mellitus, Glucose, Insulin receptor, Metabolism, Zebrafish
- MeSH Terms
-
- Animals
- Disease Models, Animal*
- Eye Proteins/metabolism
- Glucose/metabolism*
- Glycosylation
- Hyperglycemia/etiology
- Hyperglycemia/metabolism*
- Hypoglycemic Agents/therapeutic use
- Insulin/pharmacology
- Metformin/therapeutic use
- Muscle, Skeletal/metabolism
- Receptor, Insulin/metabolism
- Sulfonylurea Compounds/therapeutic use
- Zebrafish
- PubMed
- 24704522 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Capiotti, K.M., Antonioli, R., Kist, L.W., Bogo, M.R., Bonan, C.D., Da Silva, R.S. (2014) Persistent impaired glucose metabolism in a zebrafish hyperglycemia model. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 171C:58-65.
Abstract
Diabetes mellitus (DM) affects over 10% of the world's population. Hyperglycemia is the main feature for the diagnosis of this disease. The zebrafish (Danio rerio) is an established model organism for the study of various metabolic diseases. In this paper, hyperglycemic zebrafish, when immersed in a 111mM glucose solution for 14days, developed increased glycation of proteins from the eyes, decreased mRNA levels of insulin receptors in the muscle, and a reversion of high blood glucose level after treatment with anti-diabetic drugs (glimepiride and metformin) even after 7days of glucose withdrawal. Additionally, hyperglycemic zebrafish developed an impaired response to exogenous insulin, which was recovered after 7days of glucose withdrawal. These data suggest that the exposure of adult zebrafish to high glucose concentration is able to induce persistent metabolic changes probably underlined by a hyperinsulinemic state and impaired peripheral glucose metabolism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping