PUBLICATION
Preparation, antiangiogenic and antitumoral activities of the chemically sulfated glucan from Phellinus ribis
- Authors
- Liu, Y., Liu, Y., Jiang, H., Xu, L., Cheng, Y., Wang, P.G., Wang, F.
- ID
- ZDB-PUB-140513-166
- Date
- 2014
- Source
- Carbohydrate Polymers 106: 42-8 (Journal)
- Registered Authors
- Keywords
- Antiangiogenesis, Antitumoral activity, Phellinus ribis, Sulfated derivatives, β-Glucan
- MeSH Terms
-
- Angiogenesis Inhibitors/chemistry
- Angiogenesis Inhibitors/pharmacology*
- Animals
- Antineoplastic Agents/chemistry
- Antineoplastic Agents/pharmacology*
- Basidiomycota/growth & development
- Basidiomycota/metabolism*
- Carbohydrate Sequence
- Carcinoma, Hepatocellular/blood supply
- Carcinoma, Hepatocellular/drug therapy
- Carcinoma, Hepatocellular/pathology
- Cell Proliferation/drug effects
- Cells, Cultured
- Female
- Glucans/chemistry
- Glucans/pharmacology*
- Human Umbilical Vein Endothelial Cells
- Humans
- Immunoenzyme Techniques
- Liver Neoplasms/blood supply
- Liver Neoplasms/drug therapy
- Liver Neoplasms/pathology
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Neovascularization, Pathologic/prevention & control*
- Ovarian Neoplasms/blood supply
- Ovarian Neoplasms/drug therapy
- Ovarian Neoplasms/pathology
- Xenograft Model Antitumor Assays
- Zebrafish/growth & development
- Zebrafish/metabolism
- beta-Glucans/chemistry*
- PubMed
- 24721049 Full text @ Carbohydr. Polym.
Citation
Liu, Y., Liu, Y., Jiang, H., Xu, L., Cheng, Y., Wang, P.G., Wang, F. (2014) Preparation, antiangiogenic and antitumoral activities of the chemically sulfated glucan from Phellinus ribis. Carbohydrate Polymers. 106:42-8.
Abstract
Two sulfated derivatives (PRP-S1 and PRP-S2) of a β-glucan from Phellinus ribis with different degrees of substitution were obtained by chlorosulfonic acid method. The derivatives could block formation of new vessels in zebrafish and inhibit the proliferation of human umbilical vein endothelial cells (HUVECs). The two sulfated derivatives had remarkably high antitumor activities in vivo (in BALB/c mice inoculated with H22 hepatocellular carcinoma) as well as in vitro (against human ovary cancer SKOV-3 cells), without producing any overt signs of general toxicity. The results of immunohistochemistry assay indicated that the derivatives significantly reduced the average number of microvessel density (MVD) and inhibited the expression of vascular endothelial growth factor (VEGF) in tumor. Thus, these derivatives exhibit pronounced antiangiogenic and antitumoral properties. Except for cytotoxic effects on tumor cells, it is reasonable to expect that the antitumoral effects of PRP-S1 and PRP-S2 are mediated via their antiangiogenic properties.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping