ZFIN ID: ZDB-PUB-140513-151
Rapid adaptive optical recovery of optimal resolution over large volumes
Wang, K., Milkie, D.E., Saxena, A., Engerer, P., Misgeld, T., Bronner, M.E., Mumm, J., Betzig, E.
Date: 2014
Source: Nature Methods 11(6): 625-8 (Journal)
Registered Authors: Bronner-Fraser, Marianne, Mumm, Jeff, Saxena, Ankur
Keywords: none
MeSH Terms: Animals; Cell Line; Microscopy, Confocal/instrumentation*; Microscopy, Confocal/statistics & numerical data*; Optics and Photonics/instrumentation* (all 8) expand
PubMed: 24727653 Full text @ Nat. Methods
ABSTRACT
Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.
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