ZFIN ID: ZDB-PUB-140513-151
Rapid adaptive optical recovery of optimal resolution over large volumes
Wang, K., Milkie, D.E., Saxena, A., Engerer, P., Misgeld, T., Bronner, M.E., Mumm, J., Betzig, E.
Date: 2014
Source: Nature Methods   11(6): 625-8 (Journal)
Registered Authors: Bronner-Fraser, Marianne, Mumm, Jeff, Saxena, Ankur
Keywords: none
MeSH Terms:
  • Animals
  • Cell Line
  • Microscopy, Confocal/instrumentation*
  • Microscopy, Confocal/statistics & numerical data*
  • Optics and Photonics/instrumentation*
  • Optics and Photonics/standards
  • Time Factors
  • Zebrafish
PubMed: 24727653 Full text @ Nat. Methods
Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.