PUBLICATION

Generation and characterization of gsualpha:EGFP transgenic zebrafish for evaluating endocrine-disrupting effects

Authors
Cheng, X., Chen, X., Jin, X., He, J., Yin, Z.
ID
ZDB-PUB-140513-119
Date
2014
Source
Toxicology and applied pharmacology   278(1): 78-84 (Journal)
Registered Authors
He, Jiangyan, Yin, Zhan
Keywords
Endocrine disrupting, Pituitary hormone, Transgenic zebrafish, gsuα expression, gsuα promoter
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Endocrine Disruptors/toxicity*
  • Gene Expression Regulation, Developmental/drug effects
  • Genes, Reporter*
  • Glycoprotein Hormones, alpha Subunit/genetics*
  • Green Fluorescent Proteins/biosynthesis
  • Green Fluorescent Proteins/genetics*
  • Pituitary Gland/drug effects*
  • Pituitary Gland/embryology
  • Pituitary Gland/metabolism
  • Promoter Regions, Genetic/drug effects*
  • Toxicity Tests
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed
24747804 Full text @ Tox. App. Pharmacol.
Abstract
The glycoprotein subunit &alpha (gsuα) gene encodes the shared α subunit of the three pituitary heterodimeric glycoprotein hormones: follicle-stimulating hormone β (Fshβ), luteinizing hormone β (Lhβ) and thyroid stimulating hormone β (Tshβ). In our current study, we identified and characterized the promoter region of zebrafish gsuα and generated a stable gsuα:EGFP transgenic line, which recapitulated the endogenous gsuα expression in the early developing pituitary gland. A relatively conserved regulatory element set is presented in the promoter regions of zebrafish and three other known mammalian gsuα promoters. Our results also demonstrated that the expression patterns of the gsuα:EGFP transgene were all identical to those expression patterns of the endogenous gsuα expression in the pituitary tissue when our transgenic fish were treated with various endocrine chemicals, including forskolin (FSK), SP600125, trichostatin A (TSA), KClO4, dexamethasone (Dex), β-estradiol and progesterone. Thus, this gsuα:EGFP transgenic fish reporter line provides another valuable tool for investigating the lineage development of gsuα-expressing gonadotrophins and the coordinated regulation of various glycoprotein hormone subunit genes. These reporter fish can serve as a novel platform to perform screenings of endocrine-disrupting chemicals (EDCs) in vivo as well.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes