ZFIN ID: ZDB-PUB-140404-1
Proteome-Driven Elucidation of Adaptive Responses to Combined Vitamin E and C Deficiency in Zebrafish
Motorykin, I., Traber, M.G., Tanguay, R.L., and Maier, C.S.
Date: 2014
Source: Journal of Proteome Research 13(3): 1647-56 (Journal)
Registered Authors: Tanguay, Robert L.
Keywords: none
MeSH Terms:
  • Adaptation, Physiological*
  • Animals
  • Ascorbic Acid/administration & dosage
  • Ascorbic Acid/metabolism*
  • Ascorbic Acid Deficiency/metabolism*
  • Chromatography, Liquid
  • Humans
  • Mass Spectrometry/methods
  • Metabolic Networks and Pathways
  • Protein Interaction Mapping
  • Proteome/analysis*
  • Proteome/metabolism
  • Tissue Extracts/chemistry
  • Vitamin E/administration & dosage
  • Vitamin E/metabolism*
  • Vitamin E Deficiency/metabolism*
  • Zebrafish
PubMed: 24476500 Full text @ J. Proteome Res.

The purpose of this study was to determine the system-wide consequences of deficiencies in two essential micronutrients, vitamins E and C, on the proteome using zebrafish (Danio rerio) as one of the few vertebrate models that similar to humans cannot synthesize vitamin C. We describe a label-free proteomics workflow to detect changes in protein abundance estimates dependent on vitamin regimes. We used ion-mobility-enhanced data-independent tandem mass spectrometry to determine differential regulation of proteins in response to low dietary levels of vitamin C with or without vitamin E. The detection limit of the method was as low as 20 amol, and the dynamic range was five orders of magnitude for the protein-level estimates. On the basis of the quantitative changes obtained, we built a network of protein interactions that reflect the whole organism’s response to vitamin C deficiency. The proteomics-driven study revealed that in vitamin-E-deficient fish, vitamin C deficiency is associated with induction of stress response, astrogliosis, and a shift from glycolysis to glutaminolysis as an alternative mechanism to satisfy cellular energy requirements.