ZFIN ID: ZDB-PUB-140403-2
An anterior limit of FGF/Erk signal activity marks the earliest future somite boundary in zebrafish
Akiyama, R., Masuda, M., Tsuge, S., Bessho, Y., and Matsui, T.
Date: 2014
Source: Development (Cambridge, England)   141(5): 1104-1109 (Journal)
Registered Authors: Akiyama, Ryutaro, Matsui, Takaaki
Keywords: Clock, FGF signalling, Segmentation, Somitogenesis
MeSH Terms:
  • Animals
  • Body Patterning/genetics
  • Body Patterning/physiology
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/metabolism*
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology
  • Signal Transduction/genetics
  • Signal Transduction/physiology
  • Somites/cytology
  • Somites/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 24504340 Full text @ Development

Vertebrate segments called somites are generated by periodic segmentation of the anterior extremity of the presomitic mesoderm (PSM). During somite segmentation in zebrafish, mesp-b determines a future somite boundary at position B-2 within the PSM. Heat-shock experiments, however, suggest that an earlier future somite boundary exists at B-5, but the molecular signature of this boundary remains unidentified. Here, we characterized fibroblast growth factor (FGF) signal activity within the PSM, and demonstrated that an anterior limit of downstream Erk activity corresponds to the future B-5 somite boundary. Moreover, the segmentation clock is required for a stepwise posterior shift of the Erk activity boundary during each segmentation. Our results provide the first molecular evidence of the future somite boundary at B-5, and we propose that clock-dependent cyclic inhibition of the FGF/Erk signal is a key mechanism in the generation of perfect repetitive structures in zebrafish development.