PUBLICATION

Sept6 Is Required for Ciliogenesis in Kupffer's Vesicle, the Pronephros, and the Neural Tube during Early Embryonic Development

Authors
Zhai, G., Gu, Q., He, J., Lou, Q., Chen, X., Jin, X., Bi, E., and Yin, Z.
ID
ZDB-PUB-140402-8
Date
2014
Source
Molecular and cellular biology   34(7): 1310-1321 (Journal)
Registered Authors
Gu, Qilin, He, Jiangyan, Yin, Zhan
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animal Structures/embryology
  • Animal Structures/metabolism
  • Animals
  • Body Patterning/genetics
  • Body Patterning/physiology
  • Cilia/metabolism*
  • Cilia/ultrastructure
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Molecular Sequence Data
  • Neural Tube/embryology
  • Neural Tube/metabolism
  • Nodal Signaling Ligands/genetics
  • Nodal Signaling Ligands/metabolism
  • Phylogeny
  • Pronephros/embryology
  • Pronephros/metabolism
  • Septins/antagonists & inhibitors
  • Septins/genetics
  • Septins/metabolism*
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Tubulin/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
24469395 Full text @ Mol. Cell. Biol.
Abstract

Septins are conserved filament-forming GTP-binding proteins that act as cellular scaffolds or diffusion barriers in a number of cellular processes. However, the role of septins in vertebrate development remains relatively obscure. Here, we show that zebrafish septin 6 (sept6) is first expressed in the notochord and then in nearly all of the ciliary organs, including Kupffer's vesicle (KV), the pronephros, eye, olfactory bulb, and neural tube. Knockdown of sept6 in zebrafish embryos results in reduced numbers and length of cilia in KV. Consequently, cilium-related functions, such as the left-right patterning of internal organs and nodal/spaw signaling, are compromised. Knockdown of sept6 also results in aberrant cilium formation in the pronephros and neural tube, leading to cilium-related defects in pronephros development and Sonic hedgehog (Shh) signaling. We further demonstrate that SEPT6 associates with acetylated α-tubulin in vivo and localizes along the axoneme in the cilia of zebrafish pronephric duct cells as well as cultured ZF4 cells. Our study reveals a novel role of sept6 in ciliogenesis during early embryonic development in zebrafish.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes