ZFIN ID: ZDB-PUB-140317-9
Impairment of the immune system in GH-overexpressing transgenic zebrafish (Danio rerio)
Batista, C.R., Figueiredo, M.A., Almeida, D.V., Romano, L.A., and Marins, L.F.
Date: 2014
Source: Fish & shellfish immunology   36(2): 519-524 (Journal)
Registered Authors: Almeida, Daniela Volcan, Figueiredo, Marcio de Azevedo, Marins, Luis Fernando
Keywords: none
MeSH Terms:
  • Adaptive Immunity
  • Animals
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/metabolism
  • Gene Expression Regulation*
  • Growth Hormone/genetics*
  • Growth Hormone/metabolism
  • Head Kidney/growth & development
  • Head Kidney/metabolism
  • Immune System/physiopathology*
  • Immunity, Innate
  • Immunohistochemistry/veterinary
  • Thymus Gland/growth & development
  • Thymus Gland/metabolism
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/immunology*
PubMed: 24406293 Full text @ Fish Shellfish Immunol.
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ABSTRACT

Growth hormone (GH) is an important regulator of immune functions in vertebrates, and it has been intensively reported a series of stimulatory actions of this hormone over on the immune system. Within aquaculture, overexpression of GH has been considered a promising alternative for promoting higher growth rates in organisms of commercial interest. Considering the various pleiotropic effects of GH, there are still few studies that aim to understand the consequences of the excess of GH on the physiological systems. In this context, our goal was to present the effects of the overexpression of GH on immune parameters using a model of zebrafish (Danio rerio) that overexpress this hormone. The results showed that GH transgenic zebrafish had 100% of mortality when immunosuppressed with dexamethasone, revealing a prior weakening of the immune system in this lineage. Morphometric analysis of thymus and head kidney revealed a reduction in the area of these structures in transgenic zebrafish. Moreover, the phenotypic expression of CD3 and CD4 thymocytes was also depreciated in transgenic zebrafish. Furthermore, a decrease was noted in the expression of genes RAG-1 (60%), IKAROS (50%), IL-1β (55%), CD4 (60%) and CD247 (40%), indicating that development parameters, of innate and acquired immunity, are being harmed. Based on these results, it can be concluded that the excess of GH impairs the immune functions in GH transgenic zebrafish, indicating that the maintenance of normal levels of this hormone is essential for the functioning of immunological activities.

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