ZFIN ID: ZDB-PUB-131202-2
Isolation of zebrafish gonads for RNA isolation
Huang, H.Y., and Ketting, R.F.
Date: 2014
Source: Methods in molecular biology (Clifton, N.J.) : 183-194 (Chapter)
Registered Authors: Ketting, René
Keywords: zebrafish, piRNA, germline development, gonads, ovary, testis, RNA, FACS, Vasa
MeSH Terms:
  • Animals
  • Dissection/methods*
  • Female
  • Flow Cytometry
  • Genotype
  • Immunoprecipitation
  • Male
  • Ovary/cytology
  • Ovary/metabolism*
  • RNA/isolation & purification*
  • Testis/cytology
  • Testis/metabolism*
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed: 24178566 Full text @ Meth. Mol. Biol.

Piwi proteins and piRNAs are abundant in the gonads of various animal species. Gonads from different developmental stages provide us information regarding the function of piRNAs and the PIWI pathway during germline development. Here we describe methods for gonad and germ cell preparation from different developmental stages of zebrafish. We also describe how to use these gonads to purify and characterize piRNAs.