Effects of perchlorate on BDE-47-induced alteration thyroid hormone and gene expression of in the hypothalamus-pituitary-thyroid axis in zebrafish larvae
- Authors
- Zhao, X., Wang, S., Li, D., You, H., and Ren, X.
- ID
- ZDB-PUB-131122-21
- Date
- 2013
- Source
- Environmental Toxicology and Pharmacology 36(3): 1176-1185 (Journal)
- Registered Authors
- Keywords
- BDE-47, PER, HPT axis, thyroid hormone, gene expression, protein expression
- MeSH Terms
-
- Animals
- Blotting, Western
- Environmental Monitoring
- Flame Retardants/toxicity*
- Gene Expression/drug effects*
- Halogenated Diphenyl Ethers/toxicity*
- Hypothalamo-Hypophyseal System/drug effects*
- Hypothalamo-Hypophyseal System/metabolism
- Larva
- Perchlorates/pharmacology*
- Pituitary Gland/drug effects*
- Pituitary Gland/metabolism
- RNA/biosynthesis
- RNA/isolation & purification
- Real-Time Polymerase Chain Reaction
- Teratogens
- Thyroid Gland/drug effects*
- Thyroid Gland/metabolism
- Thyroid Hormones/biosynthesis*
- Thyroxine/biosynthesis
- Thyroxine/metabolism
- Triiodothyronine/biosynthesis
- Triiodothyronine/metabolism
- Zebrafish/physiology*
- PubMed
- 24177579 Full text @ Environ. Toxicol. Pharmacol.
- CTD
- 24177579
To investigate the effects of perchlorate on thyroid hormone disturbances induced by 2,22,42,4-tetrabromodiphenyl ether (BDE-47) via thyroid hormone (TH)-mediated pathways, zebrafish embryos were exposed to a combination of BDE-47 and PER from the time of fertilisation to 14 d (dpf). The whole-body content of TH and the expression of genes and proteins related to the hypothalamic–pituitary–thyroid (HPT) axis were analysed. Co-exposure to BDE-47 and PER decreased the body weight and increased malformation rates relative to the effects of exposure to only BDE-47. Compared with the exposure to BDE-47 alone, the exposure to a combination of BDE-47 (10 μg/L) and PER (3.5 mg/L) significantly up-regulated the expression of genes involved in TH synthesis (NIS and Nkx2.1a) and significantly down-regulated the expression of genes related to the regulation of the HPT axis (CRH and TSHβ). The expression of TG at the gene and protein levels was significantly up-regulated, but the expression of TTR was significantly down-regulated in the co-exposures relative to BDE-47 treated alone. In addition, the larger reduction in the T4 level resulting from exposure to the mixture of BDE-47 and PER demonstrated that PER enhanced the thyroid-disruptive effects of BDE-47. These results help to elucidate the complicated chemical interactions and the molecular mechanism of action of these two TH disruptors.