PUBLICATION

Highly conserved elements discovered in vertebrates are present in non-syntenic loci of tunicates, act as enhancers and can be transcribed during development

Authors
Sanges, R., Hadzhiev, Y., Gueroult-Bellone, M., Roure, A., Ferg, M., Meola, N., Amore, G., Basu, S., Brown, E.R., De Simone, M., Petrera, F., Licastro, D., Strahle, U., Banfi, S., Lemaire, P., Birney, E., Muller, F., and Stupka, E.
ID
ZDB-PUB-131121-2
Date
2013
Source
Nucleic acids research   41(6): 3600-3618 (Journal)
Registered Authors
Ferg, Marco, Hadzhiev, Yavor, Müller, Ferenc, Strähle, Uwe
Keywords
none
MeSH Terms
  • Animals
  • Base Sequence
  • Conserved Sequence
  • Dogs
  • Enhancer Elements, Genetic*
  • Fishes/genetics
  • Gene Expression Regulation, Developmental*
  • Gene Regulatory Networks
  • Genes, Homeobox
  • Genetic Loci
  • Genome
  • Humans
  • Mammals/genetics
  • Mice
  • Synteny
  • Transcription, Genetic
  • Urochordata/genetics*
  • Vertebrates/genetics*
PubMed
23393190 Full text @ Nucleic Acids Res.
Abstract

Co-option of cis-regulatory modules has been suggested as a mechanism for the evolution of expression sites during development. However, the extent and mechanisms involved in mobilization of cis-regulatory modules remains elusive. To trace the history of non-coding elements, which may represent candidate ancestral cis-regulatory modules affirmed during chordate evolution, we have searched for conserved elements in tunicate and vertebrate (Olfactores) genomes. We identified, for the first time, 183 non-coding sequences that are highly conserved between the two groups. Our results show that all but one element are conserved in non-syntenic regions between vertebrate and tunicate genomes, while being syntenic among vertebrates. Nevertheless, in all the groups, they are significantly associated with transcription factors showing specific functions fundamental to animal development, such as multicellular organism development and sequence-specific DNA binding. The majority of these regions map onto ultraconserved elements and we demonstrate that they can act as functional enhancers within the organism of origin, as well as in cross-transgenesis experiments, and that they are transcribed in extant species of Olfactores. We refer to the elements as ‘Olfactores conserved non-coding elements’.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes