ZFIN ID: ZDB-PUB-131119-32
Fluorescently tagged Lin7c is a dynamic marker for polarity maturation in the zebrafish retinal epithelium
Luz, M., and Knust, E.
Development of epithelial cell polarity is a highly dynamic process, and often established by the sequential recruitment of
conserved protein complexes, such as the Par or the Crumbs (Crb) complex. However, detailed insights into the refinement of
polarity and the formation of the complexes are still lacking. Here, we established fluorescently tagged Lin7c, a core member
of the Crb complex, as an ideal tool to follow development of polarity in zebrafish epithelia. We find that in gastrula stages,
RFP-Lin7c is found in the cytosol of the enveloping layer, while Pard3-GFP is already polarized at this stage. During development
of the retinal epithelium, RFP-Lin7c localization is refined from being cytosolic at 14 hours post fertilization (hpf) to
almost entirely apical in cells of the eye cup at 28 hpf. This apical Lin7c localization depends on the Crb complex members
Oko meduzy and Nagie oko. Thus, fluorescently tagged Lin7c can be used in a broad range of epithelia to follow polarity maturation
in vivo and specifically to elucidate the sequence of events determining Crb complex-mediated polarity.