PUBLICATION

Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy

Authors
Gfrerer, L., Dougherty, M., and Liao, E.C.
ID
ZDB-PUB-131115-1
Date
2013
Source
Journal of visualized experiments : JoVE   (79): e50525 (Journal)
Registered Authors
Liao, Eric
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Ethmoid Bone/chemistry
  • Ethmoid Bone/embryology*
  • Luminescent Proteins
  • Microscopy, Confocal/methods*
  • Neural Crest/chemistry
  • Neural Crest/cytology
  • Neural Crest/embryology
  • SOXE Transcription Factors/genetics
  • Time-Lapse Imaging/methods*
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics
PubMed
24121214 Full text @ J. Vis. Exp.
Abstract

Vertebrate palatogenesis is a highly choreographed and complex developmental process, which involves migration of cranial neural crest (CNC) cells, convergence and extension of facial prominences, and maturation of the craniofacial skeleton. To study the contribution of the cranial neural crest to specific regions of the zebrafish palate a sox10: kaede transgenic zebrafish line was generated. Sox10 provides lineage restriction of the kaede reporter protein to the neural crest, thereby making the cell labeling a more precise process than traditional dye or reporter mRNA injection. Kaede is a photo-convertible protein that turns from green to red after photo activation and makes it possible to follow cells precisely. The sox10: kaede transgenic line was used to perform lineage analysis to delineate CNC cell populations that give rise to maxillary versus mandibular elements and illustrate homology of facial prominences to amniotes. This protocol describes the steps to generate a live time-lapse video of a sox10: kaede zebrafish embryo. Development of the ethmoid plate will serve as a practical example. This protocol can be applied to making a time-lapse confocal recording of any kaede or similar photoconvertible reporter protein in transgenic zebrafish. Furthermore, it can be used to capture not only normal, but also abnormal development of craniofacial structures in the zebrafish mutants.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes