Masuda, S., Nakatani, Y., Ren, S., and Tanaka, M. (2013) Blue Light-Mediated Manipulation of Transcription Factor Activity In Vivo. ACS Chemical Biology. 8(12):2649-53.
We developed a novel technique for manipulating the activity of transcription factors with blue light (termed “PICCORO”) using the bacterial BLUF-type photoreceptor protein PixD. The chimeric dominant-negative T-box transcription factor No Tail formed heterologous complexes with a PixD decamer in a light-dependent manner, and these complexes affected transcription repressor activity. When applied to zebrafish embryos, PICCORO permitted regulation of the activity of the mutant No Tail in response to 472-nm light provided by a light-emitting diode.