PUBLICATION
Dynamic neuroanatomy at subcellular resolution in the zebrafish
- Authors
- Faucherre, A., and López-Schier, H.
- ID
- ZDB-PUB-131009-3
- Date
- 2014
- Source
- Methods in molecular biology (Clifton, N.J.) 1082: 187-195 (Chapter)
- Registered Authors
- Faucherre, Adele, Lopez-Schier, Hernan
- Keywords
- neuroanatomy, zebrafish, live imaging, microscopy, fluorescent protein
- MeSH Terms
-
- Animals
- Axons/metabolism
- Cell Membrane/metabolism
- Imaging, Three-Dimensional
- Intracellular Space/metabolism*
- Luminescent Proteins/genetics
- Neuroanatomy/economics
- Neuroanatomy/methods*
- Neurons, Afferent/cytology
- Time Factors
- Zebrafish/anatomy & histology*
- Zebrafish/embryology
- PubMed
- 24048935 Full text @ Meth. Mol. Biol.
Citation
Faucherre, A., and López-Schier, H. (2014) Dynamic neuroanatomy at subcellular resolution in the zebrafish. Methods in molecular biology (Clifton, N.J.). 1082:187-195.
Abstract
Genetic means to visualize and manipulate neuronal circuits in the intact animal have revolutionized neurobiology. “Dynamic neuroanatomy” defines a range of approaches aimed at quantifying the architecture or subcellular organization of neurons over time during their development, regeneration, or degeneration. A general feature of these approaches is their reliance on the optical isolation of defined neurons in toto by genetically expressing markers in one or few cells. Here we use the afferent neurons of the lateral line as an example to describe a simple method for the dynamic neuroanatomical study of axon terminals in the zebrafish by laser-scanning confocal microscopy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping