Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species
- Authors
- Rosselló, R.A., Chen, C.C., Dai, R., Howard, J.T., Hochgeschwender, U., and Jarvis, E.D.
- ID
- ZDB-PUB-130918-6
- Date
- 2013
- Source
- eLIFE 2: e00036 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Biomarkers/metabolism
- Cell Lineage
- Cell Proliferation
- Cells, Cultured
- Cellular Reprogramming*
- Chick Embryo
- Chimera
- Drosophila
- Embryonic Stem Cells/metabolism*
- Finches
- Gene Expression Regulation, Developmental
- Genotype
- Induced Pluripotent Stem Cells/metabolism*
- Karyotyping
- Kruppel-Like Transcription Factors/genetics
- Kruppel-Like Transcription Factors/metabolism
- Mice
- Octamer Transcription Factor-3/genetics
- Octamer Transcription Factor-3/metabolism
- Phenotype
- Proto-Oncogene Proteins c-myc/genetics
- Proto-Oncogene Proteins c-myc/metabolism
- Quail
- SOXB1 Transcription Factors/genetics
- SOXB1 Transcription Factors/metabolism
- Telomerase/metabolism
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Transfection
- Zebrafish
- PubMed
- 24015354 Full text @ Elife
Cells are fundamental units of life, but little is known about evolution of cell states. Induced pluripotent stem cells (iPSCs) are once differentiated cells that have been re-programmed to an embryonic stem cell-like state, providing a powerful platform for biology and medicine. However, they have been limited to a few mammalian species. Here we found that a set of four mammalian transcription factor genes used to generate iPSCs in mouse and humans can induce a partially reprogrammed pluripotent stem cell (PRPSCs) state in vertebrate and invertebrate model organisms, in mammals, birds, fish, and fly, which span 550 million years from a common ancestor. These findings are one of the first to show cross-lineage stem cell-like induction, and to generate pluripotent-like cells for several of these species with in vivo chimeras. We suggest that the stem-cell state may be highly conserved across a wide phylogenetic range.