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ZFIN ID: ZDB-PUB-130722-6
Leptin expression affects metabolic rate in zebrafish embryos (D. rerio)
Dalman, M.R., Liu, Q., King, M.D., Bagatto, B., and Londraville, R.L.
Date: 2013
Source: Frontiers in Physiology 4: 160 (Journal)
Registered Authors: Liu, Qin
Keywords: leptin-A, zebrafish, rescue, metabolic rate, oxygen consumption, carbon dioxide, microplate assay
MeSH Terms: none
PubMed: 23847542 Full text @ Front. Physiol.
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ABSTRACT

We used antisense morpholino oligonucleotide technology to knockdown leptin-(A) gene expression in developing zebrafish embryos and measured its effects on metabolic rate and cardiovascular function. Using two indicators of metabolic rate, oxygen consumption was significantly lower in leptin morphants early in development [<48 hours post-fertilization (hpf)], while acid production was significantly lower in morphants later in development (>48 hpf). Oxygen utilization rates in <48 hpf embryos and acid production in 72 hpf embryos could be rescued to that of wildtype embryos by recombinant leptin coinjected with antisense morpholino. Leptin is established to influence metabolic rate in mammals, and these data suggest leptin signaling also influences metabolic rate in fishes.

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