ZFIN ID: ZDB-PUB-130710-70
Suppression and Epigenetic Regulation of MiR-9 Contributes to Ethanol Teratology: Evidence from Zebrafish and Murine Fetal Neural Stem Cell Models
Pappalardo-Carter, D.L., Balaraman, S., Sathyan, P., Carter, E.S., Chen, W.J., and Miranda, R.C.
Date: 2013
Source: Alcoholism, clinical and experimental research 37(10): 1657-1667 (Journal)
Registered Authors:
Keywords: MiR-9, zebrafish, embryonic development, ethanol, methylation
MeSH Terms:
  • Animals
  • Epigenesis, Genetic/drug effects
  • Epigenesis, Genetic/physiology*
  • Ethanol/toxicity*
  • Fetal Stem Cells/drug effects
  • Fetal Stem Cells/physiology*
  • Gene Knockdown Techniques/methods
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs/physiology*
  • Neural Stem Cells/drug effects
  • Neural Stem Cells/physiology*
  • Random Allocation
  • Teratogenesis/drug effects
  • Teratogenesis/physiology*
  • Zebrafish
PubMed: 23800254 Full text @ Alcoholism Clin. Exp. Res.
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ABSTRACT

Background

Fetal alcohol exposure produces multiorgan defects, making it difficult to identify underlying etiological mechanisms. However, recent evidence for ethanol (EtOH) sensitivity of the miRNA miR-9 suggests one mechanism, whereby EtOH broadly influences development. We hypothesized that loss of miR-9 function recapitulates aspects of EtOH teratology.

Methods

Zebrafish embryos were exposed to EtOH during gastrulation, or injected with anti-miR-9 or nonsense control morpholinos during the 2-cell stage of development and collected between 24 and 72 hours postfertilization (hpf). We also assessed the expression of developmentally important, and known miR-9 targets, FGFR-1, FOXP2, and the nontargeted transcript, MECP2. Methylation at CpG islands of mammalian miR-9 genes was assessed in fetal murine neural stem cells (mNSCs) by methylation-specific PCR, and miRNA processing assessed by qRT-PCR for pre-miR-9 transcripts.

Results

EtOH treatment and miR-9 knockdown resulted in similar cranial defects including microcephaly. Additionally, EtOH transiently suppressed miR-9, as well as FGFR-1 and FOXP2, and alterations in miR-9 expression were correlated with severity of EtOH-induced teratology. In mNSCs, EtOH increased CpG dinucleotide methylation at the miR-9-2 locus and accumulation of pre-miR-9-3.

Conclusions

EtOH exerts regulatory control at multiple levels of miR-9 biogenesis. Moreover, early embryonic loss of miR-9 function recapitulated the severe range of teratology associated with developmental EtOH exposure. EtOH also disrupts the relationship between miR-9 and target gene expression, suggesting a nuanced relationship between EtOH and miRNA regulatory networks in the developing embryo. The implications of these data for the expression and function of mature miR-9 warrant further investigation.

ADDITIONAL INFORMATIONNo data available