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ZFIN ID: ZDB-PUB-130703-36
Zebrafish CD59 has both bacterial-binding and inhibiting activities
Sun, C., Wu, J., Liu, S., Li, H., and Zhang, S.
Date: 2013
Source: Developmental and comparative immunology   41(2): 178-188 (Journal)
Registered Authors: Li, Hongyan
Keywords: zebrafish, complement, CD59, bacterial-binding activity, antimicrobial activity
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Bacteria/drug effects
  • Bacteria/immunology*
  • Bacteria/metabolism
  • Base Sequence
  • Blotting, Western
  • Brain/embryology
  • Brain/immunology
  • Brain/metabolism
  • CD59 Antigens/classification
  • CD59 Antigens/genetics
  • CD59 Antigens/immunology*
  • Escherichia coli/drug effects
  • Escherichia coli/immunology
  • Escherichia coli/metabolism
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Hydrophobic and Hydrophilic Interactions
  • Lipopolysaccharides/immunology
  • Lipopolysaccharides/metabolism
  • Male
  • Models, Molecular
  • Molecular Sequence Data
  • Phylogeny
  • Protein Binding
  • Protein Conformation
  • Recombinant Proteins/immunology
  • Recombinant Proteins/metabolism
  • Recombinant Proteins/pharmacology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Staphylococcus aureus/drug effects
  • Staphylococcus aureus/immunology
  • Staphylococcus aureus/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/immunology*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/immunology*
PubMed: 23707788 Full text @ Dev. Comp. Immunol.

CD59, known as protectin, usually plays roles as a regulatory inhibitor of complement, but it also exhibits activities independent of its function as a complement inhibitor. This study reported the identification and characterization of an ortholog of mammalian cd59 from zebrafish Danio rerio, which is similar to known cd59 in terms of both amino acid sequence and genomic structure as well as synteny conservation. We showed that zebrafish cd59 was maternally expressed in early embryos and expressed in a tissue-specific manner, with most abundant expression in the brain. We further showed that recombinant zebrafish CD59 was capable of binding to both the Gram-negative and Gram-positive bacteria as well as the microbial signature molecules LPS and LTA. In addition we demonstrated that recombinant zebrafish CD59 displayed slight antimicrobial activity capable of inhibiting the growth of E. coli and S. aureus. All these data indicate that zebrafish CD59 can not only binds to the bacteria and their signature molecules LPS and LTA but can also inhibit their growth, a novel role assigned to CD59.