Characterization of the trunk neural crest in the bamboo shark, Chiloscyllium punctatum
- Authors
- Juarez, M., Reyes, M., Coleman, T., Rotenstein, L., Sao, S., Martinez, D., Jones, M., Mackelprang, R., and de Bellard, M.E.
- ID
- ZDB-PUB-130605-20
- Date
- 2013
- Source
- The Journal of comparative neurology 521(14): 3303-20 (Journal)
- Registered Authors
- Keywords
- shark embryo, sox8, sox9, neural crest, Chiloscyllium punctatum
- MeSH Terms
-
- Amino Acids/metabolism
- Animals
- CD57 Antigens/metabolism
- Cell Differentiation/physiology
- Cell Movement
- Gene Expression Regulation, Developmental/physiology*
- Microscopy, Electron, Scanning
- Neural Crest/cytology*
- Neural Crest/embryology*
- Neuroglia/metabolism
- Neurons/metabolism
- Phylogeny
- SOXE Transcription Factors/metabolism
- Sequence Analysis, Protein
- Sharks/anatomy & histology
- Sharks/embryology
- Tubulin/metabolism
- PubMed
- 23640803 Full text @ J. Comp. Neurol.
The neural crest is a population of mesenchymal cells that after migrating from the neural tube give rise to a structures and cell-types: jaw, part of the peripheral ganglia and melanocytes. Although much is known about neural crest development in jawed vertebrates, a clear picture of trunk neural crest development for elasmobranchs is yet to be developed. Here we present a detailed study of trunk neural crest development in the bamboo shark, Chiloscyllium punctatum. Vital labeling with DiI and in situ hybridization using cloned Sox8 and Sox9 probes demonstrated that trunk neural crest cells follow a pattern similar to the migratory paths already described in zebrafish and amphibians. We found shark trunk neural crest along the rostral side of the somites, the ventromedial pathway, branchial arches, gut, sensory ganglia and nerves. Interestingly, Chiloscyllium punctatum Sox8 and Sox9 sequences aligned with vertebrate SoxE genes, but appeared to be more ancient than the corresponding vertebrate paralogs. The expression of these two SoxE genes in trunk neural crest cells, especially Sox9, matched the Sox10 migratory patterns observed in teleosts. Interestingly, we observed DiI cells and Sox9 labeling along the lateral line, suggesting that in C. punctatum, glial cells in the lateral line are likely of neural crest origin. Though this has been observed in other vertebrates, we are the first to show that the pattern is present in cartilaginous fishes. These findings demonstrate that trunk neural crest cell development in Chiloscyllium punctatum follows the same highly conserved migratory pattern observed in jawed vertebrates.