PUBLICATION
Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio
- Authors
- Reinardy, H.C., Skippins, E., Henry, T.B., and Jha, A.N.
- ID
- ZDB-PUB-130322-32
- Date
- 2013
- Source
- Journal of Fish Biology 82(3): 1074-1081 (Journal)
- Registered Authors
- Henry, Theodore B.
- Keywords
- comet assay, DNA strand breaks, hydrogen peroxide, repeated sampling, single-cell gel electrophoresis assay
- MeSH Terms
-
- Animals
- DNA Damage*
- Hydrogen Peroxide/adverse effects
- Male
- Sperm Count
- Spermatozoa/pathology*
- Zebrafish*
- PubMed
- 23464563 Full text @ J. Fish Biol.
Citation
Reinardy, H.C., Skippins, E., Henry, T.B., and Jha, A.N. (2013) Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio. Journal of Fish Biology. 82(3):1074-1081.
Abstract
Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2O2, 0–200 μM), and in vitro exposure of sperm to 200 μM H2O2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping