PUBLICATION

Generating parabiotic zebrafish embryos for cell migration and homing studies

Authors
Demy, D.L., Ranta, Z., Giorgi, J.M., Gonzalez, M., Herbomel, P., and Kissa, K.
ID
ZDB-PUB-130218-5
Date
2013
Source
Nature Methods   10(3): 256-258 (Journal)
Registered Authors
Demy, Doris-Lou, Giorgi, Jean-Michel, Gonzalez, Magali, Herbomel, Philippe, Kissa-Marin, Karima
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Movement/physiology*
  • Cell Tracking/methods*
  • Embryo, Nonmammalian*
  • Hematopoietic Stem Cells/physiology*
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Parabiosis/methods*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/physiology
PubMed
23377378 Full text @ Nat. Methods
Abstract

Parabiosis, the surgical generation of conjoined organisms sharing a common bloodstream, has been a powerful tool for studying hematopoietic cell migration and interaction with stromal niches in rodent and avian systems. We describe a technique to generate parabiotic zebrafish embryos based on blastula fusion. This procedure permits the in vivo visualization of hematopoietic cell migration and homing to niches and peripheral tissues in zebrafish parabiotes of different genetic backgrounds.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes