Special Technical Requirements in Automated Handling and Microscopy of Living Organisms
- Authors
- Pylatiuk, C., Pfriem, A., Liebel, U., Schulz, S., and Bretthauer, G.
- ID
- ZDB-PUB-130128-3
- Date
- 2011
- Source
- at-Automatisierungstechnik 59(11): 692-698 (Journal)
- Registered Authors
- Liebel, Urban, Pylatiuk, Christian
- Keywords
- abiotic stress, zebrafish, mechanical stress, lighting, embryogenesis
- MeSH Terms
- none
- PubMed
- none Full text @ at-Automatisierungstechnik
Zebrafish were established as model organisms for biological studies of embryonic development and toxicology testing. Automated manipulation and microscopy of living organisms is needed in all process steps and several new robots have been developed in the last years. The impact of abiotic stress factors like low and high ambient temperatures on zebrafish embryo development have been quantified thoroughly in the past. However, very little is known about the impact of mechanical stress or irradiation with artficial bright daylight that occurs during manipulation and microscopy of living organisms. In this study no evidence was found for a negative impact of bright white light on zebrafish embryo development. Additionally the maximum pressure was evaluated to fixate an zebrafish egg with a vacuum gripper without doing harm to the chorion.