PUBLICATION

Ve-ptp Modulates Vascular Integrity by Promoting Adherens Junction Maturation

Authors
Carra, S., Foglia, E., Cermenati, S., Bresciani, E., Giampietro, C., Lora Lamia, C., Dejana, E., Beltrame, M., and Cotelli, F.
ID
ZDB-PUB-121221-13
Date
2012
Source
PLoS One   7(12): e51245 (Journal)
Registered Authors
Beltrame, Monica, Cotelli, Franco
Keywords
Embryos, Vascular permeability, Zebrafish, Tight junctions, Blood, Endothelial cells, Tails, Junctional complexes
MeSH Terms
  • Adherens Junctions/enzymology
  • Adherens Junctions/physiology*
  • Animals
  • Base Sequence
  • Blood Vessels/enzymology
  • Blood Vessels/physiology*
  • Blotting, Western
  • Cells, Cultured
  • DNA Primers
  • Fluorescent Antibody Technique
  • Humans
  • In Situ Hybridization
  • Neovascularization, Physiologic
  • Receptor-Like Protein Tyrosine Phosphatases, Class 3/metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish
PubMed
23251467 Full text @ PLoS One
Abstract

Background

Endothelial cell junctions control blood vessel permeability. Altered permeability can be associated with vascular fragility that leads to vessel weakness and haemorrhage formation. In vivo studies on the function of genes involved in the maintenance of vascular integrity are essential to better understand the molecular basis of diseases linked to permeability defects. Ve-ptp (Vascular Endothelial-Protein Tyrosine Phosphatase) is a transmembrane protein present at endothelial adherens junctions (AJs).

Methodology/Principal Findings

We investigated the role of Ve-ptp in AJ maturation/stability and in the modulation of endothelial permeability using zebrafish (Danio rerio). Whole-mount in situ hybridizations revealed zve-ptp expression exclusively in the developing vascular system. Generation of altered zve-ptp transcripts, induced separately by two different splicing morpholinos, resulted in permeability defects closely linked to vascular wall fragility. The ultrastructural analysis revealed a statistically significant reduction of junction complexes and the presence of immature AJs in zve-ptp morphants but not in control embryos.

Conclusions/Significance

Here we show the first in vivo evidence of a potentially critical role played by Ve-ptp in AJ maturation, an important event for permeability modulation and for the development of a functional vascular system.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes