PUBLICATION
Optogenetic control of transcription in zebrafish
- Authors
- Liu, H., Gomez, G., Lin, S., Lin, S., and Lin, C.
- ID
- ZDB-PUB-121220-2
- Date
- 2012
- Source
- PLoS One 7(11): e50738 (Journal)
- Registered Authors
- Gomez, Gustavo, Lin, Shuo
- Keywords
- none
- MeSH Terms
-
- Animals
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Cryptochromes/metabolism
- Gene Expression Regulation, Fungal/genetics
- Gene Expression Regulation, Fungal/radiation effects
- Light
- Optogenetics/methods*
- Saccharomyces cerevisiae/genetics
- Saccharomyces cerevisiae/metabolism
- Saccharomyces cerevisiae/radiation effects
- Transcription, Genetic/genetics*
- Transcription, Genetic/radiation effects*
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 23226369 Full text @ PLoS One
Citation
Liu, H., Gomez, G., Lin, S., Lin, S., and Lin, C. (2012) Optogenetic control of transcription in zebrafish. PLoS One. 7(11):e50738.
Abstract
Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2) and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1). We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping