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ZFIN ID: ZDB-PUB-121206-29
Anthrax toxin receptor 2a controls mitotic spindle positioning
Castanon, I., Abrami, L., Holtzer, L., Heisenberg, C.P., van der Goot, F.G., and González-Gaitán, M.
Date: 2013
Source: Nature cell biology   15(1): 28-39 (Journal)
Registered Authors: Heisenberg, Carl-Philipp
Keywords: none
MeSH Terms:
  • Actins/metabolism
  • Animals
  • Bacterial Proteins/biosynthesis
  • Bacterial Proteins/genetics
  • Carrier Proteins/metabolism
  • Cell Membrane/metabolism
  • Cell Polarity
  • Cytoskeleton/metabolism
  • Embryo, Nonmammalian/cytology
  • Gene Knockdown Techniques
  • Germ Layers/cytology
  • Germ Layers/metabolism
  • Luminescent Proteins/biosynthesis
  • Luminescent Proteins/genetics
  • Microtubule-Associated Proteins/metabolism
  • Mitosis
  • Monomeric GTP-Binding Proteins/metabolism
  • Monomeric GTP-Binding Proteins/physiology
  • Morpholinos/genetics
  • Neuropeptides/metabolism
  • Protein Transport
  • Receptors, Peptide/genetics
  • Receptors, Peptide/metabolism
  • Receptors, Peptide/physiology*
  • Recombinant Fusion Proteins/biosynthesis
  • Recombinant Fusion Proteins/genetics
  • Spindle Apparatus/metabolism*
  • Time-Lapse Imaging
  • Wnt Signaling Pathway
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
  • rho-Associated Kinases/metabolism
PubMed: 23201782 Full text @ Nat. Cell Biol.

Oriented mitosis is essential during tissue morphogenesis. The Wnt/planar cell polarity (Wnt/PCP) pathway orients mitosis in a number of developmental systems, including dorsal epiblast cell divisions along the animal-vegetal (A-V) axis during zebrafish gastrulation. How Wnt signalling orients the mitotic plane is, however, unknown. Here we show that, in dorsal epiblast cells, anthrax toxin receptor 2a (Antxr2a) accumulates in a polarized cortical cap, which is aligned with the embryonic A-V axis and forecasts the division plane. Filamentous actin (F-actin) also forms an A-V polarized cap, which depends on Wnt/PCP and its effectors RhoA and Rock2. Antxr2a is recruited to the cap by interacting with actin. Antxr2a also interacts with RhoA and together they activate the diaphanous-related formin zDia2. Mechanistically, Antxr2a functions as a Wnt-dependent polarized determinant, which, through the action of RhoA and zDia2, exerts torque on the spindle to align it with the A-V axis.