The Zebrafish- Danio rerio - Is a Useful Model for Measuring the Effects of Small-molecule Mitigators of Late Effects of Ionizing Irradiation
- Authors
- Epperly, M.W., Bahary, N., Quader, M., Dewald, V., and Greenberger, J.S.
- ID
- ZDB-PUB-121205-21
- Date
- 2012
- Source
- In vivo (Athens, Greece) 26(6): 889-897 (Journal)
- Registered Authors
- Bahary, Nathan
- Keywords
- radiation fibrosis, antioxidant therapy, zebrafish
- MeSH Terms
-
- Amifostine/pharmacology
- Animals
- Antioxidants/pharmacology
- Cyclic N-Oxides/pharmacology
- Dose-Response Relationship, Radiation
- Embryonic Development/radiation effects*
- Fibrosis*/physiopathology
- Fibrosis*/therapy
- Humans
- Organometallic Compounds/pharmacology
- Radiation, Ionizing*
- Radiation-Protective Agents*
- Salicylates/pharmacology
- Spin Labels
- Survival Analysis
- Zebrafish/embryology
- Zebrafish/physiology
- PubMed
- 23160669
Background/Aim: Use of zebrafish models may decrease the cost of screening new irradiation protectors and mitigators. Materials and Methods: Zebrafish (Danio rerio) models were tested for screening water-soluble radiation protectors and mitigators. Irradiation of embryos and monitoring survival, and measuring fibrosis of the caudal musculature of adults allowed for testing of acute and late effects, respectively. Results: Incubation of zebrafish embryos either before or after irradiation in ethyl pyruvate (1 mM) increased survival. Irradiation of adults to 15 to 75 Gy, delivered in single-fraction at 13 Gy/min, showed dose-dependent fibrosis at 30 days, quantitated as physiological decrease in swimming tail movement, and histopathological detection of collagen deposition in the dorsal musculature. Continuous administration of small-molecule radioprotector drugs in the water after irradiation reduced both acute and chronic injuries. Conclusion: The zebrafish is cost-effective for screening new radiation countermeasures.