PUBLICATION

High-Throughput Screening of Zebrafish Embryos Using Automated Heart Detection and Imaging

Authors
Spomer, W., Pfriem, A., Alshut, R., Just, S., and Pylatiuk, C.
ID
ZDB-PUB-121019-7
Date
2012
Source
Journal of Laboratory Automation   17(6): 435-442 (Journal)
Registered Authors
Just, Steffen, Pylatiuk, Christian
Keywords
high-throughput screening, zebrafish, heart, automated feature detection
MeSH Terms
  • Animals
  • Automation, Laboratory/methods*
  • Cardiovascular Agents/isolation & purification
  • Heart/drug effects
  • Heart/embryology*
  • High-Throughput Screening Assays*
  • Image Processing, Computer-Assisted/methods*
  • Microscopy, Video/methods*
  • Zebrafish/embryology*
PubMed
23053930 Full text @ J. Lab. Autom.
Abstract

Over the past decade, the zebrafish has become a key model organism in genetic screenings and drug discovery. A number of genes have been identified to affect the development of the shape and functioning of the heart, leading to zebrafish mutants with heart defects. The development of semiautomated microscopy systems has allowed for the investigation of drugs that reverse a disease phenotype on a larger scale. However, there is a lack of automated feature detection, and commercially available computer-aided microscopes are expensive. Screening of the zebrafish heart for drug discovery typically includes the identification of heart parameters, such as the frequency or fractional shortening. Until now, screening processes have been characterized by manual handling of the larvae and manual microscopy. Here, an intelligent robotic microscope is presented, which automatically identifies the orientation of a zebrafish in a micro well. A predefined region of interest, such as the heart, is detected automatically, and a video with higher magnification is recorded. Screening of a 96-well plate takes 35 to 55 min, depending on the length of the videos. Of the zebrafish hearts, 75% are recorded accurately without any user interaction. A description of the system, including the graphical user interface, is given.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes