PUBLICATION

The Nodal regulated dusp4 inhibits mesendoderm formation during zebrafish gastrulation

Authors
Liu, Z.T., Wei, S., and Wang, Q.
ID
ZDB-PUB-121010-5
Date
2012
Source
Yi chuan = Hereditas   34(9): 1153-1158 (Journal)
Registered Authors
Wang, Qiang
Keywords
none
MeSH Terms
  • Animals
  • Dual-Specificity Phosphatases/genetics*
  • Dual-Specificity Phosphatases/metabolism
  • Gastrulation/genetics*
  • Gene Expression Regulation, Developmental
  • Membrane Proteins/metabolism*
  • Mesoderm/embryology*
  • Mesoderm/metabolism
  • Signal Transduction
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
PubMed
23017456
Abstract

MAP kinase phosphatase-2 (MKP-2/DUSP4), a dual specificity protein phosphatase with tyrosine/serine/ threonine phosphatase activity, is associated with cellular proliferation and differentiation, but its functions during embryo development are unclear. To study the developmental function of dusp4, we first examined the spatiotemporal expression pattern of this gene during zebrafish embryonic development by whole mount in situ hybridization. We found that dusp4 was maternally expressed since its transcripts were present from the one-cell to the 256-cell stages. At early gastrulation stages, dusp4 transcripts specifically distributed at margin region, where the mesendodermal cells were located. Further-more, Nodal signal was crucial for dusp4 expression. The expression of dusp4 was obviously increased in Nodal ligand overexpressed embryos, while its expression was almost lost in the Nodal signal-deficient MZoep mutants. In addition, dusp4 MO was also designed to knock down its expression in embryos. The mesendoderm formation was significantly in-creased in dusp4 morphants, but not obviously changed in dusp4 overexpressed embryos, suggesting that dusp4 is necessary, but not sufficient for the inhibitory of mesendoderm induction. Thus, our results indicate that Nodal regulated dusp4 plays a repressive role in mesendoderm induction.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping